Concerning the molecular weight, shape, and size of polyglucose isolated from HeLa cells.

نویسندگان

  • F Sokol
  • I L Graves
  • W W Ackermann
چکیده

Light-scattering measurements were made on a preparation of polyglucose isolated from HeLa cells. The molecular weight was determined, from a Zimm plot of the data, to be 4.6" lO 6 . The character of the angular dependence of the particlescattering factor suggests the shape of the particle in solution with 779 3~ gyration radius approximates that of a rigid rod, 27oo A in length with a thickness of 5o A. By quantitative electron microscopy, using reference latex spheres, the molecular weight was estimated to be 1.35"1o 6. Electron micrographs show the preparation when air-dried on a collodion membrane to be composed of relatively uniform spheroid particles, approx. 30o A in diameter.

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عنوان ژورنال:
  • Biochimica et biophysica acta

دوره 112 1  شماره 

صفحات  -

تاریخ انتشار 1966